Cyclic voltammetry was used to characterize the ssDNA immobilization and hybridization with its complementary DNA sequences by using methylene blue as indicator. 在硬脂酸铝离子膜上进行DNA探针的固定和与目标基因的杂交。
Molecular beacons are novel fluorescence oligonucleotide probes fluorescing upon hybridization to their complementary DNA/ RNA targets with excellent sensitivity and high selectivity. 分子信标是一种高灵敏度、高特异性的新型荧光核酸探针。它在与互补DNA/RNA靶序列杂交时放出荧光。
The general strategy for cloning chymosin gene is to synthesize complementary DNA ( cDNA) by reverse transcription of mRNA fron which introns have been spliced out and then the cDNA is cloned and expressed in a suitable host. 克隆凝乳酶的一般策略是经过mRNA反转录合成cDNA,将内涵子拼接掉后把cDNA克隆在表达宿主中进行表达。
Small RNAs can guide posttranscriptional degradation of complementary messenger RNAs and, in plants, transcriptional gene silencing by methylation of homologous DNA sequences. 小RNA能诱导互补信使RNA在转录后降解,对于植物,可通过同源DNA序列甲基化使转录基因沉默。
A Study of the Hydration of Complementary Bases G-C, A-T in B-BNA Molecule BDNA分子中互补碱基对GC、AT水合作用的研究
Methods: ( 1) Reverse transcription-polymerase chain reaction ( RT-PCR) was use to obtain the TR gene complementary DNA ( cDNA). 方法:(1)进行逆转录-聚合酶链式反应(RTPCR),获得TR基因cDNA;
The optimal co-ordination mode for the hydration of complementary bases G-C, A-T in B-DNA molecule has been derived by using quantum chemical calculations and empirical potential energy functions. 本文通过对BDNA分子中互补碱基对AT、GC水合作用的量子化学计算以及最优化处理求得了它们水合作用的最优配位模式。
DNA template directs organic synthesis by sequence-specificity recruiting reactants linked to complementary DNA. DNA模板指导的有机合成反应具有序列特异性特点。
Oligonucleotides have the ability to selectively block target gene and mRNA according to base-pair complementary binding at the process of replication and transcription of DNA, cutting, transfer or translation of mRNA, and thereby may become the kind of perfect therapeutic agents at genetic level. 寡核苷酸可根据碱基互补原则特异性地与靶基因或mRNA结合,从基因复制、转录、mRNA剪接、转运或转译等水平上抑制目的基因的表达,从而实现基因调控。
Hybridization of 293-BK cell DNA with labelled BKV DNA or with complementary RNA indicates that the viral genome or BK viral DNA sequences are present in 293-BK cells. 293-BK细胞DNA与标记的BKVDNA或互补RNA的杂交试验提示,病毒基因组或BKVDNA顺序存在于293-BK细胞中。
The frequencies of four bases in coding regions almost equal to those in complementary strand. 四种碱基在产DNA双链编码区之间的使用频率相等。
The chicken growth hormone ( GH) complementary DNA ( cDNA) have been isolated from a cDNA library, which was constructed with meat-type chicken breed pituitary. 本研究从肉用品种鸡的垂体文库中克隆分离了鸡生长激素cDNA,并作了核苷酸序列测定。
Primary Study on Interaction Change of Complementary Base Pairs in DNA during H Bond Breaking ( Continued) DNA分子互补碱基对氢键解裂前后相互作用变化的初步研究(续)
It binds to complementary RNA or DNA with specificity and form a triplex complex with highly stability. 它与DNA或RNA杂交特异性很强,可与靶基因形成稳定的三螺旋结构。
Changes of nuclear RNA and polysomal RNA complementary to the non-repeated DNA sequences in rat hepatoma 与单一顺序DNA互补的大鼠肝癌核RNA和聚核糖核蛋白体RNA的变化
Inherited global search capability, DNA genetic algorithm simulates double helix structure and utilizes molecular satisfying the Waston-Crick complementary condition to enhance the validity and the speed of convergence of the genetic algorithm. So DNA genetic algorithm can solve NP-hard problem of Flow Shop scheduling efficiently. DNA遗传算法继承了遗传算法全局搜索的能力,同时利用DNA双螺旋结构和碱基互补配对原则进行编码运算,提高了算法的有效性和收敛速度,从而很好地解决了NP-hard性质的Flow-Shop调度问题。
A study of complementary DNA cloning and sequence determination of rabbit type PLA2 with potent bactericidal activity 具强杀菌活性的兔Ⅱ型磷脂酶A2互补DNA克隆及其顺序确定的研究
Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA. 新近制备了大量纯化的pEH920DNA,该质粒DNA插入了登革病毒2型核酸片段的互补DNA。
Antisense technique, developed on the principle of base complementation, is a method using short strand oligonucleotide fragments complementary to target DNAs or target RANs for inhibition of gene expression. 反义技术是根据碱基互补原理,利用与靶DNA或靶RNA互补的短链寡聚核苷酸片段抑制基因表达的方法。
Therefore, we tried to clone the complementary DNA of hBMP-3 gene by RT-PCR, for the purpose of producing human morphogenetic protein in the mammary glands of transgenic animals. 鉴于此,本研究拟通过RT-PCR的方法克隆该基因,为利用转基因动物乳腺生产人骨形成蛋白创造条件。
Fungus-transmitted wheat mosaic virus ( FWMV-C) isolated from Henan, China was used for viral RNA extraction and complementary DNA synthesis. 以采自河南的真菌传小麦花叶病毒(FWMV-C)为材料,抽提病毒RNA,合成互补DNA(CDNA)。
By measuring the amplification of RLS signal, complete complementary and bases mismatched DNA sequences can easily be detected and identified. 通过测定放大的RLS信号,完全互补和有碱基错配的DNA序列能很容易地被检测和识别。
In the absence of target DNA, complementary DNA showed the status of the single-stranded, RCA reaction was initiated by addition of the circular template, polymerase, ligase and dNTP. 在没有目标物DNA时,互补DNA呈现单链状态,其在环状模板,聚合酶,连接酶以及dNTP的存在下进行RCA扩增。
The aptamer probe has a electroactive ferrocene-labeled tail sequence that is complementary to the surface-tethered DNA strands with a predesigned low melting temperature. 该适体探针尾端修饰电活性基团二茂铁,且单个尾端序列与表面固定的短链寡核苷酸探针的熔链温度较低。
After covalently adsorbed with PO4 probe sequence, PAMAM/ MWNT-COO-/ GC electrode was immersed in the solutions of complementary sequences of different concentrations, and then we can get Rct-concentration curve by EIS. 该修饰电极共价键合DNA探针序列,在不同浓度的互补序列溶液中经过杂交反应后,检测电极表面的阻抗变化,得到Rct&浓度曲线。
Its principle is mainly in the bases of complementary matching principle. Through the formation and launching of hairpin structure in corresponding DNA chains, use gel electrophoresis operation to separate different lengths of DNA chain, and obtain the solution of the problem in the end. 其原理主要是在碱基互补配对的基础上,通过相应DNA链发夹结构的不断形成与展开,利用凝胶电泳操作将各种不同长度的DNA链分离出来,最终得到所求问题的解。